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Microphotography - how to focus the digital camera through the microscope: how to obtain sharp, in-focus photographs through the microscope using a low-cost digital camera. The purpose of this paper is to help microscopists photograph, store, and use digital microphotographs using modern digital cameras and transmitted-light microscopes.
Discussion focuses on selection of cameras adapt well to microscope eyepieces or trinocular heads, and on camera settings and procedures to obtain best quality photographs. We also discuss image resolution and size settings and make recommendations. Cameras used as examples in this paper include the Nikon Coolpix series 990, 995, and 4500.
[The photograph at page very top is one of our earliest attempts at photographing Aspergillus sp. using lacto phenol cotton blue stain. Even a beginner can obtain very good microphotographs with just a little care. The photograph shown here is of one of our stereoscopic microscopes in use for making digital photographs.
The techniques discussed in this paper work well with digital cameras and any type of microscope, low power stereo zoom to high power forensic polarized light microscopes.]
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PAAA 2005 Symposium, University of Tulsa, Tulsa Oklahoma - June 2-5, 2005 - updated 02/23/2009
Hold the camera dead still against the eyepiece while shooting; the rim of the camera lens should press against your eyepiece sides, so you're not scratching anyone's lens. Or use a standard lens mount that replaces the eyepiece, or use a microscope with a trinocular head and mount your camera there.
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