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This article describes & how to collect mold, allergen, or other particle or mold test samples from carpets, furniture, drapes, or other soft surfaces to aid in the diagnosis of the cause of interior wall, ceiling, flooring or carpeting stains and explains how to recognize their probable cause and source, including soot stains, house dust stains, pet or animal stains, and thermal tracking or thermal bridging stains associated with building air leaks, and building insulation defects.

Often these stains are mistaken for toxic indoor mold. When investigating a building for a mold problem, you can save mold test costs by learning how to recognize Stuff that is Not Mold or is only Harmless Mold but may be mistaken for more serious contamination - save your money. Because some clients have on occasion sent samples to our mold test lab that really should not have been collected, much less looked-at, we provide this library of photographs of things that are "not mold" and don't need to be tested.

These are substances that you can easily learn to recognize in buildings. Save your mold test money, and increase the accuracy of your mold contamination inspection or test for toxic or allergenic mold in buildings: review these items to learn recognize non-fungal materials or even possibly harmless cosmetic "black mold" often mistaken for "toxic fungal growth."

See STAIN DIAGNOSIS on BUILDING INTERIORS and STAINS on buildings - QUICK GUIDE and STAINS on INDOOR SURFACES: PHOTO GUIDE as well as STAINS & Thermal Tracking. See Black stains from animals for details about pet stains on building floors (urine) and walls (various) and see Pet Stains on Walls for diagnosing stains such as the black marks left by pets on walls. Readers should also see STAINS on Indoor Surfaces: PHOTO GUIDE and for outdoor stains, see Stain Diagnosis on Building Exteriors.

Also see Carpeting odors: diagnose & cure carpet smells due to mold, mildew, pet urine, and carpet stains - thermal tracking, and carpet testing advice.

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How to Collect Indoor Mold, Allergens, Dust, Debris, or Stain Particles from Carpets or Other Soft Surfaces for Microscopic Lab Analysis

How to Collect Tape Samples of Stains, Dust, or Debris on Hard Surfaces or Surfaces with Visible Mold

For particles or stains found on hard surfaces indoors such as walls, ceilings, or furniture, or where mold growth is visible on any surface including carpeting or furniture, often a simple adhesive tape sample will perform well.

Furthermore, this sampling procedure is itself diagnostic, since if the adhesive tape is unable to lift and collect any particles from the surface, that also tells us something about the type of staining present. But remember that even an apparently "clear" tape sample (when viewed by the naked eye) may contain important diagnostic particles which will be quite evident when viewed as a properly prepared microscopic sample and at proper magnification and lighting in a forensic microscope.

Caution: Surface samples of mold growth on a soft material such as floor carpets or upholstered furniture may identify what is growing on the surface but it may fail to identify what is growing inside the same material if a different mold genera or species is present in that location.

See Six Easy Steps to Get and Mail a Mold Test Kit to Our Lab for 24-Hour Analysis and Report for a surface sampling procedure using adhesive tape. You can use this sampling method to collect surface particles for submission to any qualified forensic laboratory not just ours.

Qualitative Analysis (what particle types dominate a sample) of Carpet Dust Samples

We use an air-sampling cassette connected to a vacuum pump to collect debris from carpets, upholstered furniture, or carpets. In the hands of an experienced investigator a useful non-quantitative analysis can be performed to collect particles which, examined in the lab, can tell us the dominant particles present in the debris.

The lab should also be asked to cite other particles, even occurring at low levels, if the particle type is particularly diagnostic of a potential indoor air quality problem. An example is the presence of chains of Penicillium/Aspergillus mold spores since when found occurring in chains, these spores are suggestive of a nearby (potentially toxic or allergenic) mold contamination problem.

What is the Consistency of the Vacuum Method for Testing Carpets, Couches, Curtains, or Other Surfaces for Mold or Other Particles?

As you can see in the photograph of sampling cassettes above, we may use a template to define a four square inch area to be vacuumed. While it is a simple matter to define a consistent area size when using vacuum methods to test soft surfaces such as carpets or couches, it is very very difficult to actually sample these surfaces in a manner which is quantitatively consistent from sample to sample.

While some independent studies have attempted to explore this question by "seeding" a carpet surface with a known quantity of particles, we are very doubtful of the conclusions by some investigators if they assert that sampling consistency can be achieved. Where sampling consistency cannot be achieved, the quantitative comparison of samples from different areas is a tricky business, and except for findings of the presence or absence of high levels or dominant-particle levels of certain particles in a carpet vacuum sample, our view is that more detailed findings should be viewed with caution.

Here are some sources of inconsistency in vacuum sampling of soft surfaces:

• Sample location Debris level: Variation in the amount of soil, debris, or particles in different locations on the surface to be sampled. Two successive vacuum samples of the very same spot are likely to produce different results since each sampling removes some particles. Two vacuum samples of nearby spots on a carpet may produce different results because of variations in the level of debris by area.
• Sampling Location Traffic or Cleaning Level: Vacuum sampling of a high traffic area of a carpet or vacuuming an area of carpeting which is easily reached during regular or special carpet cleaning processes may produce a very different result than a vacuum sample collected from an area exposed to (or not exposed) to settling particles, mold growth, variations in moisture level, or ease of access for ordinary cleaning
• Pile depth: Variations in pile depth of carpets or other fabric surfaces
• Moisture Levels: Variations in moisture exposure of different areas of carpeting which is suspected of mold contamination
• Particle collection efficiency: the percentage of particles captured by adhesive type sampling cassettes varies by particle size and velocity; if the sampler is not designed for collection of the particles of interest, or as is very common with vacuum samples of surfaces containing a high level of debris, if the adhesive surface becomes blocked by particles, subsequent material entering the sampler is lost, making any quantitative analysis questionable.
• Sample area size: Variations in sampling area size
• Vacuum strength: Variation in the vacuum strength of the sampling pump itself. Especially if a battery-operated pump is used, the pump strength may vary during use as battery level declines.

High Efficiency Vacuum Sampling Methods for Carpets or Other Surfaces

Other vacuum methods which use a calibrated flow rate and a sampling filter can collect nearly 100% of the particles from a surface, permitting a quantitative analysis of the number of particles per square inch. In our OPINION this is often a rather questionable procedure. Even studies which claim to report that the results of this sampling method are repeatable (a measure of sampling method reliability) we find that there is an enormous variation, probably several orders of magnitude, in the number and possibly even the type of particles collected in such samples depending on the investigator's choice of sampling location, sample duration, vacuum strength, and other factors.

The result may be an analysis which is impressive in its precision, say giving 1,243.275 particles of particle type X per square inch of surface, but completely inaccurate (because there maybe so much variability due to sampling procedure that sampling an area one foot away gives a particle level of 124,327.5 particles per square inch.).

See PHOTO GUIDE TO STAINS on Indoor Surfaces for examples of some common types of indoor stains on HVAC registers, doors, carpets.

Usually soot marks, thermal bridging, or thermal tracking stains appear, if at all, in the building interior locations listed just below discussed in the remaining sections of this article.

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• Additional technical contributors & reference sources for this article are listed below.
• Thanks to Alan Carson, Carson Dunlop, Associates, Toronto, for technical critique and content suggestions regarding thermal tracking.
• We solicit more thermal tracking, soot tracking, air bypass leaks, and similar photos of indoor stains as well as text suggestions to expand this detail and would be glad to credit contributors.

Use links just below or at the left of each page to navigate this document or to view other topics at this website. Green links show where you are in our document or website.

THERMAL TRACKING & THERMAL BRIDGING
DIAGNOSE OTHER DARK STAINS on Indoor Surfaces
STAINS & FINISHES, INTERIOR
STAINS on INDOOR SURFACES: PHOTO GUIDE
CARPET DUST IDENTIFICATION
CARPET MOLD CONTAMINATION
CARPET PADDING ASBESTOS, MOLD, ODORS
CARPET STAIN DIAGNOSIS
CARPET & other STAIN TESTS
CARPET TEST GUIDE
CABINETS & COUNTERTOPS
CARPETING & INDOOR AIR QUALITY
CARPETING, SELECTION & INSTALLATION
also see INDOOR HOUSE DUST & DEBRIS

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

• Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.

• "The Elimination of Unsafe Guardrails, a Progress Report," Elliott O. Stephenson, Building Standards, March-April 1993
• "Are Functional Handrails Within Our Grasp" Jake Pauls, Building Standards, January-February 1991
• Access Ramp building codes:
  • UBC 1003.3.4.3
  • BOCA 1016.3
  • ADA 4.8.2
  • IBC 1010.2
• Access Ramp Standards:
  • ADA (Americans with Disabilities Act), Public Law 101-336. 7/26/90 is very often cited by other sources for good design of stairs and ramps etc. even where disabled individuals are not the design target.
  • ANSI A117.4 Accessible and Usable buildings and Facilities (earlier version was incorporated into the ADA)
  • ASTM F 1637, Standard Practice for Safe Walking Surfaces, (Similar to the above standards)
• America's Favorite Homes, mail-order catalogues as a guide to popular early 20th-century houses, Robert Schweitzer, Michael W.R. Davis, 1990, Wayne State University Press ISBN 0814320066 (may be available from Wayne State University Press)
• American Plywood Association, APA, "Portland Manufacturing Company, No. 1, a series of monographs on the history of plywood manufacturing",Plywood Pioneers Association, 31 March, 1967, www.apawood.org
• Animal Allergens: Dog, Cat, and Other Animal Dander - Cleanup & Prevention Information for Asthmatics and regarding Indoor Air Quality.
• Asbestos: How to find and recognize asbestos in buildings - visual inspection methods, list of common asbestos-containing materials
• Asbestos HVAC Ducts and Flues field identification photos and guide
• Asbestos products and their history and use in various building materials such as asphalt and vinyl flooring includes discussion which draws on Asbestos, Its Industrial Applications, D.V. Rosato, engineering consultant, Newton, MA, Reinhold Publishing, 1959 Library of Congress Catalog Card No.: 59-12535 (out of print).
• Asbestos Identification and Testing References
• Asbestos Identification, Walter C.McCrone, McCrone Research Institute, Chicago, IL.1987 ISBN 0-904962-11-3. Dr. McCrone literally "wrote the book" on asbestos identification procedures which formed the basis for current work by asbestos identification laboratories.
• Stanton, .F., et al., National Bureau of Standards Special Publication 506: 143-151
• Pott, F., Staub-Reinhalf Luft 38, 486-490 (1978) cited by McCrone
• Building Research Council, BRC, nee Small Homes Council, SHC, School of Architecture, University of Illinois at Urbana-Champaign, brc.arch.uiuc.edu. "The Small Homes Council (our original name) was organized in 1944 during the war at the request of the President of the University of Illinois to consider the role of the university in meeting the demand for housing in the United States. Soldiers would be coming home after the war and would be needing good low-cost housing. ...  In 1993, the Council became part of the School of Architecture, and since then has been known as the School of Architecture-Building Research Council. ... The Council's researchers answered many critical questions that would affect the quality of the nation's housing stock.
  • How could homes be designed and built more efficiently?
  • What kinds of construction and production techniques worked well and which did not?
  • How did people use different kinds of spaces in their homes?
  • What roles did community planning, zoning, and interior design play in how neighborhoods worked
• "An Example of Colonial Paneling", Norman Morrison Isham, The Metropolitan Museum of Art Bulletin, Vol. 6, No. 5 (May, 1911), pp. 112-116, available by JSTOR.
• Dust from the World Trade Center collapse following the 9/11/01 attack: the lower floors of this building contained spray-on fire-proofing asbestos materials.
• "Energy Savers: Whole-House Supply Ventilation Systems [copy on file as /interiors/Energy_Savers_Whole-House_Supply_Vent.pdf ] - ", U.S. Department of Energy energysavers.gov/your_home/insulation_airsealing/index.cfm/mytopic=11880?print
• "Energy Savers: Whole-House Exhaust Ventilation Systems [copy on file as /interiors/Energy_Savers_Whole-House_Exhaust.pdf ] - ", U.S. Department of Energy energysavers.gov/your_home/insulation_airsealing/index.cfm/mytopic=11870
• "Energy Savers: Ventilation [copy on file as /interiors/Energy_Savers_Ventilation.pdf ] - ", U.S. Department of Energy
• "Energy Savers: Natural Ventilation [copy on file as /interiors/Energy_Savers_Natural_Ventilation.pdf ] - ", U.S. Department of Energy
• "Energy Savers: Energy Recovery Ventilation Systems [copy on file as /interiors/Energy_Savers_Energy_Recovery_Venting.pdf ] - ", U.S. Department of Energy energysavers.gov/your_home/insulation_airsealing/index.cfm/mytopic=11900
• "Energy Savers: Detecting Air Leaks [copy on file as /interiors/Energy_Savers_Detect_Air_Leaks.pdf ] - ", U.S. Department of Energy
• "Energy Savers: Air Sealing [copy on file as /interiors/Energy_Savers_Air_Sealing_1.pdf ] - ", U.S. Department of Energy
• Falls and Related Injuries: Slips, Trips, Missteps, and Their Consequences, Lawyers & Judges Publishing, (June 2002), ISBN-10: 0913875430 ISBN-13: 978-0913875438
  "Falls in the home and public places are the second leading cause of unintentional injury deaths in the United States, but are overlooked in most literature. This book is unique in that it is entirely devoted to falls. Of use to primary care physicians, nurses, insurance adjusters, architects, writers of building codes, attorneys, or anyone who cares for the elderly, this book will tell you how, why, and when people will likely fall, what most likely will be injured, and how such injuries come about. "
• Fiberglass: Indoor Air Quality Investigations: Health Concerns About Airborne Fiberglass: Fiberglass in Indoor Air from HVAC ducts, and Building Insulation
• Humidity: What indoor humidity should we maintain in order to avoid a mold problem?
• Lighting, proper use of: proper aiming of a good flashlight can disclose hard to see but toxic light or white mold colonies on walls.
• Pergo AB, division of Perstorp AB, is a Swedish manufacturer or modern laminate flooring products. Information about the U.S. company can be found at http://www.pergo.com where we obtained historical data used in our discussion of the age of flooring materials in buildings.
• Plank House Construction: weblog from plankhouse.wordpress.com/2009/01/25/plank-house-construction/ and where plank houses were built by native Americans, see
  Large 1:6 Scale Plank House Construction / P8094228, Photographer: Mike Meuser
  06/12/2007 documented at yurokplankhouse.com where scale model Museum quality Yurok Plank Houses are being sold to raise money for the Blue Creek - Ah Pah Traditional Yurok Village project.
• Re-Bath, tub lining products is a bath tub relining manufacturer and distributor located in Tempe, Arizona - see rebath.com
• Slips, Trips, Missteps and Their Consequences, Second Edition, Gary M. Bakken, H. Harvey Cohen,A. S. Hyde, Jon R. Abele, ISBN-13: 978-1-933264-01-1 or ISBN 10: 1-933264-01-2, available from the publisher, Lawyers ^ Judges Publishing Company,Inc., www.lawyersandjudges.com sales@lawyersandjudges.com and also from the InspectAPedia Bookstore (Amazon.com)
• The Stairway Manufacturers' Association, (877) 500-5759, provides a pictorial guide to the stair and railing portion of the International Residential Code. [copy on file as http://www.stairways.org/pdf/2006%20Stair%20IRC%20SCREEN.pdf ] -
• What Mold and Allergens Look Like: mold identification photos to help identify mold - choosing what to sample in buildings
• How to Clean Moldy Wood Framing & Sheathing How to clean/seal mold from/on exposed lumber or plywood subfloor or roof sheathing indoors - some suggestions based on our field and laboratory research
• Lighting, proper use of: proper aiming of a good flashlight can disclose hard to see but toxic light or white mold colonies on walls.
• Manufactured & Modular Homes: Modular Building Systems Association, MBSA, modularhousing.com, is a trade association promoting and providing links to contact modular builders in North America. Also see the Manufactured Home Owners Association, MHOAA, at www.mhoaa.us. The Manufactured Home Owners Association of America is a National Organization dedicated to the protection of the rights of all people living in Manufactured Housing in the United States.
• Mold spores in the Home - a Photo ID Library for detection and identification of mold allergens.
• How to Find and Test For Mold in buildings A "how to" photo and text primer on finding and choosing the right spots to test for mold in buildings
• Stuff that is not mold but is often mistaken for it - things you may not want to test. Also, not all "black mold" is toxic - here are examples of harmless black mold.
• Simple Adhesive Tape Sampling of Moldy Surfaces - how to send a mold sample to our lab
• Mold Sampling Methods in the Indoor Environment - In-depth article: detailed critique of popular mold testing methods - Is your mold test kit worth the bother?
• Mold-Resistant Building Practices, advice from an expert on how to prevent mold after a building flood and how to prevent mold growth in buildings by selection of building materials and by anti-mold construction details.
• Slips, Trips, Missteps and Their Consequences, Gary M. Bakken, H. Harvey Cohen, Jon R. Abele, Alvin S. Hyde, Cindy A. LaRue, Lawyers and Judges Publishing; 2 edition (April 2006), ISBN-10: 1933264012 ISBN-13: 978-1933264011
• Steps and Stairways, Cleo Baldon & Ib Melchior, Rizzoli, 1989.
• The Staircase, Ann Rinaldi
  
• Common Sense Stairbuilding and Handrailing, Fred T. Hodgson
• The Art of Staircases, Pilar Chueca
• Building Stairs, by pros for pros, Andy Engel
• A Simplified Guide to Custom Stairbuilding, George R. Christina
• Basic Stairbuilding, Scott Schuttner
  
• The Staircase (two volumes), John Templar, Cambridge: the MIT Press, 1992
• The Staircase: History and Theories, John Templar, MIT Press 1995
• Steps and Stairways, Cleo Baldon & Ib Melchior, Rizzoli, 1989.
• "The Dimensions of Stairs", J. M. Fitch et al., Scientific American, October 1974.
• "Weather-Resistive Barriers [copy on file as /interiors/Weather_Resistant_Barriers_DOE.pdf ] - ", how to select and install housewrap and other types of weather resistive barriers, U.S. DOE
• Weaver: Beaver Board and Upson Board: Beaver Board and Upson Board: History and Conservation of Early Wallboard, Shelby Weaver, APT Bulletin, Vol. 28, No. 2/3 (1997), pp. 71-78, Association for Preservation Technology International (APT), available online at JSTOR.
• What Style Is It?: A Guide to American Architecture, Rev., John C. Poppeliers, S. Allen Chambers, Wiley; Rev Sub edition (October 6, 2003), ISBN-10: 0471250368, ISBN-13: 978-0471250364
• ...