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This article discusses Mold or Airborne Particle Levels versus Air Sampler Height when testing for airborne mold. This document is a brief tutorial which provides information about the accuracy of and sources of errors in tests for the level of allergenic and toxic mold in residential buildings: Are spore counts valid? Are cultures and swab tests valid? These critical questions are discussed in this paper.

Readers should also see MOLD LEVEL IN AIR, VALIDITY, and for a more in-depth critique of popular mold testing methods than this tutorial see Mold Sampling Methods in the Indoor Environment

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Variation in Airborne Particle Levels due to Placement Height of Air Sampling Device

How much difference does it make whether the air sampling machine is placed on top of or underneath a mold-contaminated table? The photographs above show microphotographs of representative sections of an airborne particle sample trace in the same room in a building, with a single variation: the left hand photograph of a mold spore sample shows just a few fungal spores was collected with our sample placed on top of a basement pool table.

The right hand photo of a mold spore trap sample shows a very high spore concentration in the same room, obtained when we placed our sampler on the floor below the (mold infected) pool table. In both samples we used passive methods.

Our photos above show a game table left in a basement where an airborne mold problem was suspected. The table looked "clean" but inspecting below (see our flashlight leaning on the left table leg?) shows thick yellow and green mold growth on the un-sealed chipboard structure.

Had we stirred room air (say by turning on a fan or waving a notebook, it is likely that these "under table" mold spores would have been distributed more uniformly in the building's indoor air and that they would have been present in an air sample at a much higher level than with passive spore trap air sampler use.

Many IAQ consultants place their air sampler at about chest or head height in a building, presuming that will best represent the particles that will be inhaled by building occupants. This sounds reasonable except that mechanical disturbance of local indoor dust will cause enormous variation in the actual particle level at any given time and will probably make the particle distribution more uniform in the space.

Our field and lab experiments show one to three orders of magnitude (or more) variation in airborne spore counts at the same location depending on the use of passive or aggressive sampling methods.

How many mold reports document the details of sampling such as where mold was visible, where the sampler was placed, and what possible variations in airborne particle level might obtain depending on details of how the sample was collected such as whether fans are turned on or off, the room was occupied or empty, windows open or shut, vacuum cleaners running, children playing nearby, etc. Virtually never is this information considered, gathered, or reported by the industrial hygienist or other mold investigator in residential indoor air quality investigations.

How to Interpret Airborne Mold Tests in View of Air Sampling Inaccuracy

We conclude that if an indoor air test for mold such as using a spore trap or air sampling device detects a high level of problem mold spores (or other airborne particles) we believe that those results have meaning and indicate a problem - although that type of test alone is faulty because it is not prescriptive: we still have not identified the source of the problem so we still do not know what action is needed.

But if an indoor air test for mold does not detect a high level of airborne mold or other problem particles, we cannot rely on this procedure alone to reach a confident conclusion about the air quality in the building.

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ACCURACY OF AIRBORNE MOLD SPORE COUNTS
  Causes of Variation in Airborne Particle Levels
  Indoor vs. Outdoor Spore Counts
  Extent of Variation of Airborne Particle Counts
  Particle Levels vs Sampler Height
  Particle Levels vs Windows/Doors
  PARTICLE & MOLD LEVELS in DUCTWORK
  Concentration Bursts of Mold Spores
  False Negative Results in Mold Tests
  Mold Culture Plate Test Errors
  Why Use Airborne Mold/Particle Sampling?
  Visual Inspection and History for Mold

• Thanks to Susan Flappan, Flappan Consulting, moldetect.com, Overland Park KS, 913-402-1131, for contributing comments and some suggested text from ACGIH Bioaerosols: Assessment and Remediation 12/2006.

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

• Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
• Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
• Adkins and Adkins Dictionary of Roman Religion discusses Robigus, the Roman god of crop protection and the legendary progenitor of wheat rust fungus.
• Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
• "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
• US EPA - Mold Remediation in Schools and Commercial Building US EPA
• US EPA - Una Breva Guia a Moho - Hongo - en Espanol

Mold Contamination Testing, Cleanup, Prevention: references & products

• The Mold Information Center: What to Do About Mold in Buildings, When and How to Inspect for Mold, Clean Up Mold, or Avoid Mold Problems
• Aerobiology, Building Science, Microscopy, & Laboratory References, an extensive technical bibliography
• Allergens: what they look like in buildings
• Associations: Sick House, Sick Building, SBS - Air Quality, Government, Private Associations and Information Resources
• Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon)
• Atlas of Mold Related Illness: Index of Symptoms and health, physical, neurological, psychological, and other complaint which people suspect may be mold or building-related.
• Atlas of Indoor Mold, Online Clinical Mold Atlas, Toxins, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
• Black Mold that is Harmless Photos of recognizable, usually harmless black mold on wood, bluestain, ceratocystis, ophistoma
• Building Floods: quick steps after a building flood or plumbing leak can prevent costly mold contamination
• Classes of Mold: what types of cosmetic, allergenic, or toxic mold are a problem? Can mold be cleaned-up successfully?
• Clinical Mold References - Detailed bibliography of mold reference texts
• "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
• "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
• "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
  Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
• "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
• Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
• Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
• Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
• Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
• Looking for Mold Procedure: what mold is often found where in buildings - simple technical presentation
• Meruliporia: the house eating fungus or "poria"
• Mold Action Guide: Step-by-Step Instructions, What to do about mold, mildew, and other indoor allergens
• MOLD APPEARANCE - WHAT MOLD LOOKS LIKE Photos of what mold looks like in buildings
• MOLD APPEARANCE - STUFF THAT IS NOT MOLD Photos of NOT-mold material that is sometimes mistaken for mold
• MOLD ATLAS & PARTICLES INDEX, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
• MOLD BY MICROSCOPE Mold under the microscope - photo identification of the most common indoor molds found in buildings
• Mold FAQs Answers to Most Questions about Indoor Mold, Mold Related Illness, Mold Cleanup, Mold Prevention
• US EPA: Mold Remediation in Schools and Commercial Building US EPA
• Mold spores in the Home - a Photo ID Library for detection and identification of mold allergens
• Mold Test Kits - How to Collect and Send Your Own Mold Sample to our mold testing lab or to any mold lab you wish
• Most Common Indoor Molds Found in Buildings, A Table of
• Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
• Ozone Warnings - Use of Ozone as a "mold" remedy is ineffective and may be dangerous.
• Rot concerns in buildings-some building mold such as Meruliporia incrassata "Poria" risks serious rot and hidden structural damage
• US EPA: Una Breva Guia a Moho - Hongo - en Espanol

OTHER IAQ ISSUES: How To Find and Address Other Indoor Air or Indoor Environment Contaminants Besides Mold

Mold or allergens may not be the only or even the main indoor environmental contaminant. Don't let media attention to mold cause so much enviro-scare fear that other, possibly more urgent hazards go un-addressed.

• Fiberglass building insulation and HVAC duct work insulation hazards
• Sewage and Septic backup contamination in buildings: inspection, testing, remediation, & references to expert sources
• Other environmental risks: Asbestos, carbon monoxide, electromagnetic fields, environmental illness, fiberglass, MCS - multiple chemical sensitivity, toxic gases, etc
• Indoor Gas Sampling Plan for Residential Buildings lists a number of toxic indoor gases which we test for, depending on the building complaint and building conditions
• Ozone Warnings - Use of Ozone as a "mold" remedy is ineffective and may be dangerous.
• Pet control - if you can't say goodbye to your bird, cat, dog, guinea pig, hamster, tropical fish, then limit the areas they occupy and limit the airflow from that area to sleeping or other areas of the building, use allergenic bedding, eliminate wall-to-wall carpeting, improve housecleaning including use of a HEPA-rated vacuum cleaner. For more details see our article Dog, Cat, and Other Animal Dander - Information for Asthmatics and Indoor Air Quality
• Rodents, Mice, Squirrel Control - I find high levels of mouse and rodent dander, fecal dust, and urine-contaminated dust in some buildings, and high levels of these materials in building insulation in those locations. If you have a mouse problem, particularly if mice and their waste (fecals or urine) are contaminating the building HVAC or building insulation, may need both steps to clean up or remove infected materials and steps to stop an ongoing rodent problem. If squirrels are a problem, the cleanup needs to include closing off entry openings into the building. Get some help from a licensed pest control expert.
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