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MOLD: A COMPLETE GUIDE to TEST CLEAN PREVENT

AIR CLEANER PURIFIER TYPES
AIR FILTERS for HVAC SYSTEMS
AIR POLLUTANTS, COMMON INDOOR
AIR QUALITY IMPROVEMENT STRATEGIES
ALLERGEN TESTS for BUILDINGS
ANIMAL ALLERGENS / PET DANDER
ANIMAL ODORS IN BUILDINGSS
ATTORNEYS and EXPERT WITNESSES

BASEMENT MOLD
BATHROOM MOLD
BIBLIOGAPHY ENVIRONMENTAL HEALTH, MOLD, IAQ
BIOLOGICAL POLLUTANTS

CAR MOLD CONTAMINATION
CARPET DUST IDENTIFICATION
CARPET MOLD CONTAMINATION
CAT DANDER in BUILDINGS
COMBUSTION GASES & PARTICLE HAZARDS
CONDENSATION or SWEATING PIPES, TANKS
CPSC Indoor Air Pollution Book Online Copy

DUCT SYSTEM & DUCT DEFECTS

EMERGENCY RESPONSE, IAQ, GAS, MOLD

FIBERGLASS HAZARDS
FLOOD DAMAGE ASSESSMENT, SAFETY & CLEANUP
FLOODS IN BUILDINGS-mold
FORMALDEHYDE HAZARDS

GAS EXPOSURE EFFECTS, TOXIC

HUMIDITY CONTROL & TARGETS INDOORS
HOUSE DUST ANALYSIS

INDOOR AIR QUALITY IMPROVEMENT GUIDE
INSULATION MOLD

MILDEW REMOVAL & PREVENTION
MOISTURE CONTROL in BUILDINGS
MOLD GROWTH on SURFACES, TABLE OF
MYCOPHOBIA, STAINS MISTAKEN for MOLD

NOISE / SOUND DIAGNOSIS & CURE

ODORS GASES SMELLS, DIAGNOSIS & CURES
OZONE HAZARDS
OZONE for MOLD OR ODORS

RADON HAZARD TESTS & MITIGATION

SAFETY HAZARDS GUIDE
SICK HOUSE IAQ QUESTIONNAIRE
SMELL PATCH TEST to Track Down Odors
STAIN DIAGNOSIS on BUILDING INTERIORS

VENTILATION in BUILDINGS

More Information

Alternaria mold spores and hyphae from an indoor surface (C) Daniel FriedmanUsing Vacuum Cassettes or Spore Traps to Collect Mold or Particle Dust Samples
How to do it & Guide to Good Dust Sampling Practices
     

  • Field testing vacuum cassettes to collect building dust as a screen for toxic or allergenic mold contamination indoors
  • Guide to use of vacuum cassettes to screen building soft surfaces, furniture, carpeting
  • Guide to use of vacuum cassettes to collect multiple dust samples in buildings
  • MOLD CULTURE TEST KIT VALIDITY - separate article
  • SWAB & PCR SAMPLING & TESTS for MOLD - separate article
  • TAPE & BULK SAMPLING & TESTS for MOLD - separate article
  • VACUUM CASSETTE FILTER SAMPLE TESTS for DUST / MOLD
    • VACUUMING BUILDING CAVITIES - separate article
    • VACUUMING BUILDING INSULATION - separate article
    • VACUUM SAMPLING EQUIPMENT, DIY - separate article
  • Questions & answers about using vacuum sampling methods to test for building dust or mold contamination
  • References

Click to Show or Hide Related Topics

  • ACCURACY OF VARIOUS MOLD TEST METHODS - home
  • ACCURACY vs PRECISION of MEASUREMENTS
  • AIR TEST SAMPLING CASSETTE STUDY
  • AIRBORNE MOLD COUNT NUMBER GUIDE
  • AIRBORNE MOLD COUNT VALIDITY
  • AIRBORNE PARTICLE ANALYSIS METHODS
  • CONCENTRATION BURSTS of Mold Spores
  • CULTURE PLATE Test Errors, Mold
  • DUST SAMPLING PROCEDURE
  • FALSE NEGATIVEW Results in Mold Tests
  • FORENSIC & IAQ FIELD IAQ EQUIPMENT SOP - home
  • FORENSIC & IAQ LAB MICROSCOPE TECHNIQUES
  • FORENSIC LAB TECHNICAL PROCEDURES
  • HIDDEN MOLD, HOW TO FIND
  • INDOOR vs OUTDOOR Airborne Spore Counts
  • MOLD LEVEL IN AIR, VALIDITY
  • MOLD STANDARDS
  • MOLD TESTING METHOD VALIDITY
  • PARTICLE & MOLD LEVELS in DUCTWORK
  • SLIDE PREPARATION, MICROSCOPE
  • VALIDITY of MOLD TESTING METHODS
  • VARIATION IN AIRBORNE Particle Levels, Causes of
  • VARIATION IN AIRBORNE Particle Levels vs Sampler Height
  • VARIATION IN AIRBORNE PARTICLE COUNTS, Extent of
InspectAPedia tolerates no conflicts of interest. We have no relationship with advertisers, products, or services discussed at this website.

Vacuum cassette test methods for building dust or mold: this article explains the advantages and shortcomings of using vacuum cassettes or spore traps to collect mold test samples (or other dust or particle samples) from indoor surfaces such as carpets, couches, or multiple hard surface dust samples. In this article series discuss the validity of nearly all of the popular mold testing methods currently in use, pointing out the strengths and weakness of each approach to mold sampling in the indoor environment, beginning with air sampling for airborne mold levels indoors. Because mold test validity and mold test accuracy are often confused, readers should also see ACCURACY OF VARIOUS MOLD TEST METHODS. People who need to conduct mold inspection and testing indoors should see MOLD TEST PROCEDURES and TECHNICAL & LAB PROCEDURES

Green links show where you are. © Copyright 2013 InspectAPedia.com, All Rights Reserved. Author Daniel Friedman.

A Guide to Using Vacuum Samples for Screen Buildings for Toxic Mold

Portable hand vacuum (C) Daniel Friedman15th Annual North Carolina/South Carolina
Environmental Information Association Technical Conference
Myrtle Beach, SC
Daniel Friedman 23 September 2005, Updated 4/14/2009 & 10/5/2012

A collection canister is connected to an air or vacuum pump which is used to draw particles onto a filter-surface or into a special collection container.

A collection device, slide, cassette, or tape are used with a calibrated air pump to collect surface particles.

The lab prepares a slide from the cassette (of the types below) or if an MCE filter cassette was used to collect particles, the lab clears the filter onto a microscope slide, washes the filter onto a microscope slide, or uses another method to transfer particles for examination by microscope for preparation by culture.

Our MOLD INFORMATION CENTER includes more broad discussions of the overall approach to building investigation, as do many expert references cited at that web. For a more comprehensive collection information about mold test methods see INDOOR AIR QUALITY METHODS COMPARED. For more on "mold classes" (Cosmetic mold vs. allergenic mold vs. toxic or pathogenic mold) see MOLD CLASSES, HAZARD LEVELS and more references such as a Mold Action Guide are at the end of this document.

Carpet vacuum test (C) Daniel Friedman

Using a simple portable pump calibrated to a known flow rate allows rough estimation of particle density per square inch of surface tested if that analysis is needed.

People using this approach may make use of a disposable paper square template that defines a precise surface area to be vacuumed (photo above left). In our opinion a precise quantitative approach to surface vacuuming is silly because there is normally large particle variation over building surfaces for many reasons. But the approach is useful to screen for high levels of particular particles (such as mold spores, animal dander, insect allergens).

Our photo (left) shows five vacuum test areas on a hallway carpet during a study conducted by the author to examine the variation in particle deposition by foot traffic in a residential hallway.

Our hypothesis was that more outside dirt and debris could always be found in the center of the main path of foot traffic even though that area also received more aggressive vacuum cleaning than the hallway sides. We have tested carpeting before and after various types of cleaning and after suffering various types of contamination.

Surface vacuuming for mold

Spore traps (C) Daniel FriedmanWe emphasize that in this article we are discussing surface vacuuming to collect particles from an exposed surface onto which the vacuum device and collector can be placed directly.

This is an effective and useful particle or mold sampling method.

We are not referring building wall or ceiling cavity vacuuming methods that attempt to draw air and particles from the building cavity through a punctured opening and tube into a collecting device - an approach that our test found was ineffective.

We discuss building cavity vacuuming below at Vacuuming building cavities.

 

Couch test for mold or allergens (C) Daniel FriedmanVacuum samples can be useful for testing soft goods (clothing, bedding, curtains, carpets) for high levels of contaminated spores in a qualitative approach. We particularly like vacuuming a number of surfaces in an area using a single collection device as a less-costly way to make a more confident inspection of the level of contamination by moldy dust in buildings with a known problem.

We also use this method as part of a mold clearance inspection to evaluate the thoroughness of both the containment system and the general cleaning effort. For example we may collect a sample of vacuumed surface dust from 10 different surfaces in 5 rooms on a floor of a home, forming a more broad screen for moldy dust than single tape lifts of surface dust.

We've found wide variety in levels of mold found growing in or on carpets, depending on a number of variables including even the level of other dirt present in the carpeting. Some experts question this measure. Carpet vacuuming for mold is interesting as a pre and post remediation baseline data source for areas out of the remediation/containment area, but for any carpet this method quickly overloads a particle sampler.

Burkard air sampler (C) Daniel Friedman

The Burkard personal air sampler (photo at left) can also be used to vacuum particles from surfaces provided that a strong air flow is not required to lift the particles from the surface (this device pumps at 10 lpm).

Shortcomings of surface and carpet vacuuming for mold

  1. Vacuuming will not collect identifying structural components of mold as well as tape and will almost certainly damage or destroy the structures which it collects, imposing some limits on identification

  2. Vacuuming will not collect all of the material on a hard surface (which tape handles well). Particles which are easily lifted by the airflow into the canister will be over-represented compared with sticky particles which are adhered to the test surface.

    This problem is particularly sensitive to the flow rate (LPM) used. A low-flow rate (1LPM) avoids a sample overload problem (too many particles, can't read the sample) but may fail to collect or under-collect certain particles. A high flow rate improves particle pick-up but then limits the number of sample sites (increasing test cost) in order to avoid sample overload. We suspect that no vacuum method we have tested could reliably pull mold or debris reliably from deep inside a heavy upholstered couch.

  3. Carpet vacuums and some furniture or drapery vacuums will either be overloaded or restricted to culture (to which we have already objected).

Frequently Asked Questions (FAQs) about using vacuum sampling to collect dust or particles from building carpets, fabrics, furniture, insulation or other surfaces

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Technical Reviewers & References

Related Topics, found near the top of this page suggest articles closely related to this one.

  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • US EPA - Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • "IgG Food Allergy Testing by ELISA/EIA, What do they really tell us?" Sheryl B. Miller, MT (ASCP), PhD, Clinical Laboratory Director, Bastyr University Natural Health Clinic - ELISA testing accuracy: Here is an example of Miller's critique of ELISA http://www.betterhealthusa.com/public/282.cfm - Townsend Letter for Doctors and Patients
    The critique included in that article raises compelling questions about IgG testing assays, which prompts our interest in actually screening for the presence of high levels of particles that could carry allergens - dog dander or cat dander in the case at hand.
    http://www.tldp.com/issue/174/IgG%20Food%20Allergy.html contains similar criticism in another venue but interestingly by the same author, Sheryl Miller. Sheryl Miller, MT (ASCP), PhD, is an Immunologist and Associate Professor of Basic and Medical Sciences at Bastyr University in Bothell, Washington. She is also the Laboratory Director of the Bastyr Natural Health Clinic Laboratory.
  • Allergens: Testing for the level of exposure to animal allergens is discussed at http://www.animalhealthchannel.com/animalallergy/diagnosis.shtml (lab animal exposure study is interesting because it involves a higher exposure level in some cases
  • Allergens: WebMD discusses allergy tests for humans at webmd.com/allergies/allergy-tests
  • Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon) - The Atlas of Clinical Fungi is also available on CD ROM
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
  • US EPA: Mold Remediation in Schools and Commercial Building [Copy on file at /sickhouse/EPA_Mold_Remediation_in_Schools.pdf ] - US EPA
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • US EPA: Una Breva Guia a Moho - Hongo [Copy on file as /sickhouse/EPA_Moho_Guia_sp.pdf - en Espanol
  • Mark Cramer Inspection Services Mark Cramer, Tampa Florida, Mr. Cramer is a past president of ASHI, the American Society of Home Inspectors and is a Florida home inspector and home inspection educator. (727) 595-4211 mark@BestTampaInspector.com

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Home Reference Book - Carson DunlopThe Home Reference Book - the Encyclopedia of Homes, Carson Dunlop & Associates, Toronto, Ontario, 25th Ed., 2012, is a bound volume of more than 450 illustrated pages that assist home inspectors and home owners in the inspection and detection of problems on buildings. The text is intended as a reference guide to help building owners operate and maintain their home effectively. Field inspection worksheets are included at the back of the volume. Special Offer: For a 10% discount on any number of copies of the Home Reference Book purchased as a single order. Enter INSPECTAHRB in the order payment page "Promo/Redemption" space. InspectAPedia.com editor Daniel Friedman is a contributing author.

    Or choose the The Home Reference eBook for PCs, Macs, Kindle, iPad, iPhone, or Android Smart Phones. Special Offer: For a 5% discount on any number of copies of the Home Reference eBook purchased as a single order. Enter INSPECTAEHRB in the order payment page "Promo/Redemption" space.

  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
  • GO TO Carson Dunlop's Home Study Course Information - How to Become a Home Inspector: Carson Dunlop's nationally recognized Home Study Course, selected by ASHI the American Society of Home Inspectors and other professionals and associations. This website author is a contributor to this course.
    Building inspection education & report writing systems from Carson, Dunlop & Associates Ltd
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • ...

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