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ENVIRONMENTAL HAZARDS - INSPECT, TEST, REMEDY

ACCURACY OF VARIOUS MOLD TEST METHODS

AIR POLLUTANTS, COMMON INDOOR
AIR TEST FOR MOLD: ACCURACY
AIR TEST SAMPLING CASSETTE STUDY

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BIBLIOGAPHY for ENVIRONMENTAL HEALTH, MOLD, IAQ

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FLOOD DAMAGE ASSESSMENT, SAFETY & CLEANUP

MOLD: A COMPLETE GUIDE TO MOLD

MOLD INFORMATION CENTER
ACCURACY OF VARIOUS MOLD TEST METHODS
  Causes of Variation in Airborne Particle Levels
  Indoor vs. Outdoor Spore Counts
  Extent of Variation of Airborne Particle Counts
  Particle Levels vs Sampler Height
  Particle Levels vs Windows/Doors
  PARTICLE & MOLD LEVELS in DUCTWORK
  Concentration Bursts of Mold Spores
  False Negative Results in Mold Tests
  Mold Culture Plate Test Errors
  Why Use Airborne Mold/Particle Sampling?
  Visual Inspection and History for Mold
  VALIDITY of MOLD TESTING METHODS

ACTION GUIDE - WHAT TO DO ABOUT INDOOR MOLD

AIR POLLUTANTS, COMMON INDOOR
AIR TEST FOR MOLD: ACCURACY
AIR TEST SAMPLING CASSETTE STUDY
AIRBORNE MOLD SPORE COUNT ACCURACY
ALLERGEN TESTS for BUILDINGS
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BUY PRODUCTS for MOLD & ALLERGY CONTROL
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DO IT YOURSELF MOLD CLEANUP
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DUST SAMPLING PROCEDURE

ENERGY SAVINGS in BUILDINGS

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FUNGICIDAL SPRAY & SEALANT USE GUIDE

HIDDEN MOLD, HOW TO FIND
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INDOOR AIR QUALITY METHODS COMPARED

LEAD POISONING HAZARDS GUIDE
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MILDEW in BUILDINGS ?
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MOISTURE CONTROL in BUILDINGS

MOLD: A COMPLETE GUIDE TO MOLD
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MOLD ACTIVITY in BUILDINGS
MOLD AGE - Old is the Mold?

MOLD APPEARANCE - WHAT MOLD LOOKS LIKE
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MOLD BY MICROSCOPE
MOLD in the PETRI DISH, PHOTOS
Mold on Books, Book Conservation

MOLD CLASSES, HAZARD LEVELS
MOLD CLEANERS - WHAT TO USE
MOLD CLEANUP COMPANIES
MOLD CLEANUP GUIDE- HOW TO GET RID OF MOLD
MOLD CLEANUP by MEDIA BLASTING
MOLD CLEARANCE INSPECTIONS
MOLD CLINICAL REFERENCE TEXTS
MOLD CONSULTANTS/INSPECTORS
MOLD CONTAMINATION LEVELS
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MOLD DETECTION & INSPECTION GUIDE
MOLD DOCTORS - ENVIRONMENTAL MEDICINE

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MOLD BY MICROSCOPE
MOLD in the PETRI DISH, PHOTOS

MOLD ODORS, MUSTY SMELLS
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MOLD RELATED ILLNESS GUIDE
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MOLD TEST KITS
MOLD TEST KITS for DIY MOLD TESTS
MOLD TEST METHODS, ACCURACY
MOLD TEST PROCEDURES
MOLD TEST REASONS
  1. Save Money if it's Just Cosmetic Mold
  2. Mold Related Illness
  3. Mold Cleanup Data baseline
MOLD "TESTING" vs. MOLD "PROBLEM IDENTIFICATION"
  Are Mold Test Kits Useful?
MOLD TESTING METHOD VALIDITY
  Air samples
    Shortcomings of air sampling
    Mold in Air: Quantitative Analysis
  Tape sampling for mold
    Determination of mold genera
    Determination of mold species
    Shortcomings of tape sampling
  Vacuum samples
    Surface vacuuming
    Shortcomings of surface and carpet vacuuming
    Vacuuming building cavities

    Vacuuming exposed insulation
    Shortcomings of vacuuming insulation
  Cultures to "Test for Mold"
    Shortcomings of culturing
  Swab sampling
  Shortcomings of swab sampling
  PCR methods for Mold Identification
MOLD TESTING SERVICES

MSDS Material Safety Data Sheets
MVOCs & MOLDY MUSTY ODORS
MYCOTOXIN EFFECTS of MOLD EXPOSURE

Nanomaterials Hazards
NOISE / SOUND DIAGNOSIS & CURE

ODORS GASES SMELLS, DIAGNOSIS & CURE
RENTERS & TENANTS GUIDE TO MOLD

SAFETY HAZARDS GUIDE
SEPTIC SYSTEM INSPECT DIAGNOSE REPAIR
SICK HOUSE IAQ QUESTIONNAIRE
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Wall vacuum test (C) Daniel FriedmanVacuuming Building Cavities to Screen for Hidden Mold: Guide to Good Practices
InspectAPedia®  -         

  • Field testing vacuum tests of building cavities to check for mold contamination
  • Does vacuuming wall or ceiling cavities for mold produce reliable results?
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This article explains the advantages and shortcomings of using vacuum cassettes or spore traps to collect mold test samples (or other dust or particle samples) from building wall and ceiling cavities. In this article series discuss the validity of nearly all of the popular mold testing methods currently in use, pointing out the strengths and weakness of each approach to mold sampling in the indoor environment, beginning with air sampling for airborne mold levels indoors. Because mold test validity and mold test accuracy are often confused, readers should also see ACCURACY OF VARIOUS MOLD TEST METHODS. People who need to conduct mold inspection and testing indoors should see MOLD TEST PROCEDURES and TECHNICAL & LAB PROCEDURES.

Our MOLD INFORMATION CENTER includes more broad discussions of the overall approach to building investigation, as do many expert references cited at that web. For a more comprehensive collection information about mold test methods see INDOOR AIR QUALITY METHODS COMPARED. For more on "mold classes" (Cosmetic mold vs. allergenic mold vs. toxic or pathogenic mold) see MOLD CLASSES, HAZARD LEVELS and more references such as a Mold Action Guide are at the end of this document.

© Copyright 2012 InspectAPedia.com, All Rights Reserved. Information Accuracy & Bias Pledge is at below-left. Use page top links to major topics or use links at the left of each page to navigate within topics and documents at this website. Green links show where you are in a document series or at this website.

Validity/Accuracy of Vacuuming Building Cavities for a Hidden Mold Screen

15th Annual North Carolina/South Carolina
Environmental Information Association Technical Conference
Myrtle Beach, SC
Daniel Friedman 23 September 2005
, Updated 4/14/2009

Vacuuming building cavities is a popular screening practice to look for mold reservoirs. The investigator is trying to explore wall cavities while doing minimal or no visible damage.

A wall-check™ system has been marketed for this purpose and several manufacturers have coped the basic idea: a receiving Air-o-Cel™ or MCE filter cassette is attached at its inlet side to a tube which is inserted as a probe into a wall cavity, permitting creation of only a small hole. The outlet or pump side of the test device is connected to a pump and operated, typically at 15 lpm. Where we have tested this method we have experimented with both passive collection (what most investigators use) or aggressive collection (banging on the wall/ceiling at various points to attempt to dislodge and stir particles).

Short duration samples, 1-2 minutes using an Air-o-Cell ™ permit a comparatively large number of samples to be collected in a reasonably short interval. Long duration samples, perhaps for up to two hours, are collected using an MCE filter cassette.

Wall Cavity Vacuuming - Found Ineffective

Working with Louis Relle, a Louisiana mold remediation expert on a New Orleans Building which was to be demolished we collected wall-check samples from every suspect Building cavity.

When the building interior surfaces were demolished we then performed a visual inspection and collected bulk surface samples using tape. The wall check samples were completely unable to detect large and significant mold contamination in the cavities of this building.

We postulate that even with mechanical agitation (banging on the wall during wall check sampling) the flow rate of the sampling method does not move enough air to reliably pick up surface contamination unless the mold genera/species happens to be at a particularly high state of active sporulation. The tool remains in the professional's arsenal, to be used with discretion.

Shortcomings of vacuuming building cavities through a tube but our testing strongly suggests that this method is very unreliable for characterizing wall contents. We do not believe that enough air movement is created in the wall cavity (sucking any lpm flow through a small diameter tube) to reliably collect what could be a severe mold reservoir that happens not to be right next to the probe. Further if the cavity is insulated there will be virtually no air or particle movement except from very close to the probe.

What should we make of wall vacuum test results done without other inspection and testing?

Wall cavity vacuuming (C) Daniel FriedmanWall cavity vacuum tests, depending on how and when they are performed, can be like searching for a needle in a haystack while looking through a straw: if you find evidence of a problem you're lucky. But if you don't find evidence, when using a very limited-scope method, that doesn't mean that a problem is not there.

Our photo, left, is not showing the wall-check vacuum method that relies on vacuuming into a cassette through a hole in the wall. Instead, here we have pulled loose paneling and have inserted our spore trap cassette into the cavity.

This position combined with aggressive sampling by banging on the wall paneling with a flashlight is more likely to collect problem particles if they are present close to the point of vacuum cassette insertion into the wall. But even this approach is not a reliable characterization of mold risk in the building. And it is not looking into the wall cavity itself - rather we are looking at the two surfaces behind the paneling.

Wall vacuum test (C) Daniel FriedmanShort vacuum pump duration for microscopic examination (to avoid sample overload, e.g. 2-=3 minutes on an Air-O-Cell) does not move enough air to reliably find what may be in the wall cavity. An experiment done with Louis Relle in New Orleans LA demonstrated that wall-vac tests found less than 10% of large problem mold cavities that could be discovered by cutting drywall openings in a building.

Our photo (left) shows a short-duration but larger surface area or wall opening vacuuming system invented and tested by the author - it did not provide useful results.

Longer vacuum pump duration samples for viable sampling (2 hours into an MCE cassette for culturing) still may not move enough air to sample through a cavity, particularly if the cavity is insulated. Further, two-hour samples means that most-likely very few sample points were collected, making the inspection scope extremely limited and thus overall confidence in the accuracy of the picture of the building lower.

Consider that the popularly-marketed version of this wall cavity vacuuming approach to test for hidden mold contamination in buildings relies on culture of the sample. Did you know that only about 10% of molds grow in any culture at all? You're 90% uncertain of the accuracy of the test at the outset. One can't be sure that the mold that grew in the culture represents the dominant problem mold or whether it's just a low-occurrence (in the building) spore that liked the media (in the culture). We like cultures for further genera/species identification of samples but we are nervous about relying on them to tell me if the building has a problem or not.

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  • Additional technical contributors & reference sources for this article are listed below.

Use links just below or at the left of each page to navigate this document or to view other topics at this website. Green links show where you are in our document or website.

MOLD TESTING METHOD VALIDITY
Introduction
  Air samples
    Shortcomings of air sampling
    Mold in Air: Quantitative Analysis
  Tape sampling for mold
    Determination of mold genera
    Determination of mold species
    Shortcomings of tape sampling
  Vacuum samples
    Surface vacuuming
    Shortcomings of surface and carpet vacuuming
    Shortcomings of vacuuming insulation
    Vacuuming building cavities
    Vacuuming exposed insulation
  Cultures to "Test for Mold"
    Shortcomings of culturing
  Swab sampling
  Shortcomings of swab sampling
  PCR methods for Mold Identification
  Mold "Testing" vs. Mold "Problem Identification"
  Are Mold Test Kits Useful?
  Reasons to Test for or Identify Mold
    1. Save Money if it's Just Cosmetic Mold
    2. Mold Related Illness
    3. Mold Cleanup Data baseline

  • Mark Cramer Inspection Services Mark Cramer, Tampa Florida, Mr. Cramer is a past president of ASHI, the American Society of Home Inspectors and is a Florida home inspector and home inspection educator. (727) 595-4211 mark@BestTampaInspector.com

Books & Articles on Building & Environmental Inspection, Testing, Diagnosis, & Repair

  • Our recommended books about building & mechanical systems design, inspection, problem diagnosis, and repair, and about indoor environment and IAQ testing, diagnosis, and cleanup are at the InspectAPedia Bookstore. Also see our Book Reviews - InspectAPedia.
  • Environmental Health & Investigation Bibliography - our technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Adkins and Adkins Dictionary of Roman Religion discusses Robigus, the Roman god of crop protection and the legendary progenitor of wheat rust fungus.
  • Kansas State University, department of plant pathology, extension plant pathology web page on wheat rust fungus: see http://www.oznet.ksu.edu/path-ext/factSheets/Wheat/Wheat%20Leaf%20Rust.asp
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • US EPA - Mold Remediation in Schools and Commercial Building US EPA
  • US EPA - Una Breva Guia a Moho - Hongo - en Espanol

Allergies, Allergens, Allergy Testing in Buildings - References & Products

  • Air Conditioning System Blower Fans & Filters Cascading for Optimum Indoor Air Quality
  • Allergen Tests in Buildings advice about how to test, what to look for, in evaluating the level of dog, cat, or other animal allergens in a building
  • "IgG Food Allergy Testing by ELISA/EIA, What do they really tell us?" Sheryl B. Miller, MT (ASCP), PhD, Clinical Laboratory Director, Bastyr University Natural Health Clinic - ELISA testing accuracy: Here is an example of Miller's critique of ELISA http://www.betterhealthusa.com/public/282.cfm - Townsend Letter for Doctors and Patients
    The critique included in that article raises compelling questions about IgG testing assays, which prompts our interest in actually screening for the presence of high levels of particles that could carry allergens - dog dander or cat dander in the case at hand.
    http://www.tldp.com/issue/174/IgG%20Food%20Allergy.html contains similar criticism in another venue but interestingly by the same author, Sheryl Miller. Sheryl Miller, MT (ASCP), PhD, is an Immunologist and Associate Professor of Basic and Medical Sciences at Bastyr University in Bothell, Washington. She is also the Laboratory Director of the Bastyr Natural Health Clinic Laboratory.
  • Allergens: Testing for the level of exposure to animal allergens is discussed at http://www.animalhealthchannel.com/animalallergy/diagnosis.shtml (lab animal exposure study is interesting because it involves a higher exposure level in some cases
  • Allergens: WebMD discusses allergy tests for humans at webmd.com/allergies/allergy-tests
  • Animal Allergens: Dog, Cat, and Other Animal Dander - Cleanup & Prevention Information for Asthmatics and regarding Indoor Air Quality.
  • Atlas of Mold Related Illness Symptoms & Complaints - long list of both documented, studied mold related illness, and complaints ascribed to mold contamination or allergens in buildings
  • Cat Dander: how to inspect and test a building for past or current presence of cats, cat hair, cat dander, and cat allergens
  • Clinical Atlas of Mold Toxicity - An Online Description of Toxic, Pathogenic, Allergenic Fungi, Fungal Diseases
  • Fiberglass Insulation Contains Mold© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation
  • Humidity: What indoor humidity should we maintain in order to avoid a mold problem?
  • Mold Action Guide detailed guide on finding, removing, and preventing indoor mold contamination
  • Odors, Odor Detection, Smells, & Gases how to find and identify sources of noxious or toxic odors and gases
  • Other environmental risks, Our much longer list: Asbestos, carbon monoxide, electromagnetic fields, etc.
  • Ozone: The Use of Ozone Indoors for Control of Odors and Mold Removal in Buildings: A Summary of Hazards and False Claims.
  • Pollen Allergens: identification, plant pollen and indoor air quality
  • Products to Reduce Mold & Allergy Problems to reduce indoor mold or allergen levels: air cleaners, air purifiers, dust mite covers, vacuum cleaners, crawl space vents
  • Recognizing Allergens: What various indoor allergens look like - identification photos to help identify pollen, dust mites, animal dander, toxic or allergenic mold - Common Mold and other Allergens, Irritants, Remedies & Advice
  • Rodent control issues, including dander, fecal, and urine contamination of Buildings and Building insulation are discussed at our
  • Sewage and Septic backup contamination in Buildings: inspection, testing, remediation, & references to expert sources
  • Action Guide: What to do about mold, mildew, and other indoor allergens
  • ...

Mold Contamination Testing, Cleanup, Prevention: references & products

  • GO TO the MOLD and INDOOR ENVIRONMENT INFORMATION CENTER for in-depth advice on avoiding testing for or cleaning up mold and other indoor environmental hazards, odors, gases, contaminantsThe Mold Information Center: What to Do About Mold in Buildings, When and How to Inspect for Mold, Clean Up Mold, or Avoid Mold Problems
  • Aerobiology, Building Science, Microscopy, & Laboratory References, an extensive technical bibliography
  • Allergens: what they look like in buildings
  • Associations: Sick House, Sick Building, SBS - Air Quality, Government, Private Associations and Information Resources
  • Atlas of Clinical Fungi, 2nd Ed., GS deHoog, J Guarro, J Gene, & MJ Figueras, Centraalbureau voor Schimmelcultures, Universitat Rovira I Virgili, 2000, ISBN 90-70351-43-9 (you can buy this book at Amazon)
  • Atlas of Mold Related Illness: Index of Symptoms and health, physical, neurological, psychological, and other complaint which people suspect may be mold or building-related.
  • Atlas of Indoor Mold, Online Clinical Mold Atlas, Toxins, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
  • Black Mold that is Harmless Photos of recognizable, usually harmless black mold on wood, bluestain, ceratocystis, ophistoma
  • Building Floods: quick steps after a building flood or plumbing leak can prevent costly mold contamination
  • Classes of Mold: what types of cosmetic, allergenic, or toxic mold are a problem? Can mold be cleaned-up successfully?
  • Clinical Mold References - Detailed bibliography of mold reference texts
  • "A Brief Guide to Mold, Moisture, and Your Home", U.S. Environmental Protection Agency US EPA - includes basic advice for building owners, occupants, and mold cleanup operations. See http://www.epa.gov/mold/moldguide.htm
  • "Disease Prevention Program for Certain Vegetable Crops," David B. Langston, Jr., Extension Plant Pathologist - Vegetables, University of Georgia (PDF document) original source: www.reeis.usda.gov/web/crisprojectpages/209797.html
  • "Disease Prevention in Home Vegetable Gardens," Patricia Donald, Department of Plant Microbiology and Pathology, Lewis Jett
    Department of Horticulture, University of Missouri Extension - extension.missouri.edu/publications/DisplayPub.aspx?P=G6202
  • "Management of Powdery Mildew, Leveillula taurica, in Greenhouse Peppers," Ministry of Agriculture and Lands, British Columbia - Original source: www.agf.gov.bc.ca/cropprot/peppermildew.htm
  • Environmental Health & Investigation Bibliography - our own technical library on indoor air quality inspection, testing, laboratory procedures, forensic microscopy, etc.
  • Fiberglass: Mold in Fiberglass Insulation© 2005 comments about a field study in process, & more about health hazards from fiberglass insulation - DJF
  • Fifth Kingdom, Bryce Kendrick, ISBN13: 9781585100224, is available from the InspectAPedia online bookstore - we recommend the CD-ROM version of this book. This 3rd/edition is a compact but comprehensive encyclopedia of all things mycological. Every aspect of the fungi, from aflatoxin to zppspores, with an accessible blend of verve and wit. The 24 chapters are filled with up-to-date information of classification, yeast, lichens, spore dispersal, allergies, ecology, genetics, plant pathology, predatory fungi, biological control, mutualistic symbioses with animals and plants, fungi as food, food spoilage and mycotoxins.
  • Fungi, Identifying Filamentous, A Clinical Laboratory Handbook, Guy St-Germain, Richard Summerbell, Star Publishing, 1996, ISBN 0-89863-177-7 (English) (buy at Amazon)
  • Looking for Mold Procedure: what mold is often found where in buildings - simple technical presentation
  • Meruliporia: the house eating fungus or "poria"
  • Mold Action Guide: Step-by-Step Instructions, What to do about mold, mildew, and other indoor allergens
  • MOLD APPEARANCE - WHAT MOLD LOOKS LIKE Photos of what mold looks like in buildings
  • MOLD APPEARANCE - STUFF THAT IS NOT MOLD Photos of NOT-mold material that is sometimes mistaken for mold
  • MOLD ATLAS & PARTICLES INDEX, Pathogens, Allergens and Other Indoor Particles - Medical Health Effects of Mold (separate online document)
  • MOLD BY MICROSCOPE Mold under the microscope - photo identification of the most common indoor molds found in buildings
  • Mold FAQs Answers to Most Questions about Indoor Mold, Mold Related Illness, Mold Cleanup, Mold Prevention
  • US EPA: Mold Remediation in Schools and Commercial Building US EPA
  • Mold spores in the Home - a Photo ID Library for detection and identification of mold allergens
  • Mold Test Kits - How to Collect and Send Your Own Mold Sample to our mold testing lab or to any mold lab you wish
  • Most Common Indoor Molds Found in Buildings, A Table of
  • Mycology, Fundamentals of Diagnostic, Fran Fisher, Norma B. Cook, W.B. Saunders Co. 1998, ISBN 0-7216-5006-6 (buy this book at Amazon)
  • Ozone Warnings - Use of Ozone as a "mold" remedy is ineffective and may be dangerous.
  • Rot concerns in buildings-some building mold such as Meruliporia incrassata "Poria" risks serious rot and hidden structural damage
  • US EPA: Una Breva Guia a Moho - Hongo - en Espanol

OTHER IAQ ISSUES: How To Find and Address Other Indoor Air or Indoor Environment Contaminants Besides Mold

Mold or allergens may not be the only or even the main indoor environmental contaminant. Don't let media attention to mold cause so much enviro-scare fear that other, possibly more urgent hazards go un-addressed.

  • Fiberglass building insulation and HVAC duct work insulation hazards
  • Sewage and Septic backup contamination in buildings: inspection, testing, remediation, & references to expert sources
  • Other environmental risks: Asbestos, carbon monoxide, electromagnetic fields, environmental illness, fiberglass, MCS - multiple chemical sensitivity, toxic gases, etc
  • Indoor Gas Sampling Plan for Residential Buildings lists a number of toxic indoor gases which we test for, depending on the building complaint and building conditions
  • Ozone Warnings - Use of Ozone as a "mold" remedy is ineffective and may be dangerous.
  • Pet control - if you can't say goodbye to your bird, cat, dog, guinea pig, hamster, tropical fish, then limit the areas they occupy and limit the airflow from that area to sleeping or other areas of the building, use allergenic bedding, eliminate wall-to-wall carpeting, improve housecleaning including use of a HEPA-rated vacuum cleaner. For more details see our article Dog, Cat, and Other Animal Dander - Information for Asthmatics and Indoor Air Quality
  • Rodents, Mice, Squirrel Control - I find high levels of mouse and rodent dander, fecal dust, and urine-contaminated dust in some buildings, and high levels of these materials in building insulation in those locations. If you have a mouse problem, particularly if mice and their waste (fecals or urine) are contaminating the building HVAC or building insulation, may need both steps to clean up or remove infected materials and steps to stop an ongoing rodent problem. If squirrels are a problem, the cleanup needs to include closing off entry openings into the building. Get some help from a licensed pest control expert.
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